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Figure 1 Expression of PATs and protein palmitoylation in osteoblasts.A. RT PCR analysis of the mRNA levels of the 24 known PATs during osteoblast differentiation. Primary osteoblasts were cultured in the differentiation medium for different periods of time. Total RNA was isolated from these cultures and was then used to carry out RT PCR. These PATs were classified into unaltered expression (A), increased expression (B), and decreased expression (C), in comparison to the day 1 cultures. D. 2BP was able to inhibit protein palmitoylation in primary osteoblasts. Primary osteoblasts were treated with 100 M of 2BP for 2 hrs and then 3H palmitic acid was included in the culture medium for 24 hrs. The cells were harvested and the same amounts of total proteins were loaded for control and 2BP treated samples. The SDS PAGE gel was dried and exposed to x ray films. The palmitoylated proteins are radio labeled. Actin was used as a loading control..

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Protein Palmitoylation Regulates Osteoblast Differentiation through BMP Induced Osterix Expression
PLoS ONE. 2009 Jan 6; 4(1):e4135

Abstract

Osteoporosis is one of the most common diseases and can be treated by either anti resorption drugs, anabolic drugs, or both. To search for anabolic dr.....
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Leong et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Keywords

ALP

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Expression of PATs and protein palmitoylation in osteoblasts...

Article : Protein Palmitoylation Regulates Osteoblast Differentiation through BMP Induced Osterix Expression
PLoS ONE. 2009 Jan 6; 4(1):e4135

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Article : Protein Palmitoylation Regulates Osteoblast Differentiation through BMP Induced Osterix Expression
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Article : Protein Palmitoylation Regulates Osteoblast Differentiation through BMP Induced Osterix Expression
PLoS ONE. 2009 Jan 6; 4(1):e4135

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Article : Protein Palmitoylation Regulates Osteoblast Differentiation through BMP Induced Osterix Expression
PLoS ONE. 2009 Jan 6; 4(1):e4135

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Article : Protein Palmitoylation Regulates Osteoblast Differentiation through BMP Induced Osterix Expression
PLoS ONE. 2009 Jan 6; 4(1):e4135

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Article : Protein Palmitoylation Regulates Osteoblast Differentiation through BMP Induced Osterix Expression
PLoS ONE. 2009 Jan 6; 4(1):e4135

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Article : Protein Palmitoylation Regulates Osteoblast Differentiation through BMP Induced Osterix Expression
PLoS ONE. 2009 Jan 6; 4(1):e4135

Click to view details