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Figure 2 Inhibition of protein palmitoylation impeded osteoblast differentiation.A. The effect of 2BP on ALP staining. Primary osteoblasts were treated with increasing amounts of 2BP for 4 days in differentiation medium and then stained for ALP. B. Quantitation of ALP activities that were normalized to the protein levels of the cells. C. The effect of 2BP on the expression of several osteoblast differentiation markers. The experiments were carried out like Fig. 2A and total RNA were isolated from these cells. RT PCR was carried out to determine the mRNA levels of these markers. The value of control (lane 1) was set at 1.00. D. The effect of 2BP on bone nodule formation. The experiments were carried out like Fig. 2A. After 21 days in culture, the plates were stained using a Von Kossa method..

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Protein Palmitoylation Regulates Osteoblast Differentiation through BMP Induced Osterix Expression
PLoS ONE. 2009 Jan 6; 4(1):e4135

Abstract

Osteoporosis is one of the most common diseases and can be treated by either anti resorption drugs, anabolic drugs, or both. To search for anabolic dr.....
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Leong et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Keywords

ALP

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Article : Protein Palmitoylation Regulates Osteoblast Differentiation through BMP Induced Osterix Expression
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Article : Protein Palmitoylation Regulates Osteoblast Differentiation through BMP Induced Osterix Expression
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Article : Protein Palmitoylation Regulates Osteoblast Differentiation through BMP Induced Osterix Expression
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Click to view details