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Figure 3 PAT inhibitor show minimal effect on proliferation/survival of osteoblast cultures.A. Primary osteoblast cells were cultured in differentiation medium with different amounts of 2BP for 4 days and the cell numbers in each culture plates were determined by trypan blue exclusion methods. B. The experiments were done like Fig. 3A and the cells were collected and lysed in the same volume of lysis buffer and the protein concentrations were determined by the BioRad method. C. Recovery of 2BP treated cells in ALP expression. Primary osteoblasts were treated with different concentrations of 2BP for three days, washed off, and further cultured in differentiation medium for 4 more days before being stained for ALP (bottom panel), in comparison to the cells that were treated with 2BP all the time (upper panel)..

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Protein Palmitoylation Regulates Osteoblast Differentiation through BMP Induced Osterix Expression
PLoS ONE. 2009 Jan 6; 4(1):e4135

Abstract

Osteoporosis is one of the most common diseases and can be treated by either anti resorption drugs, anabolic drugs, or both. To search for anabolic dr.....
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Leong et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Keywords

ALP

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Article : Protein Palmitoylation Regulates Osteoblast Differentiation through BMP Induced Osterix Expression
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